Discovery Proteomics and Epiproteomics Analysis, Service option: Data interpretation

Overview: Analysis of histone modifications to study how chromatin architecture influences gene expression. This includes the examination of histone deacetylases (HDACs), crucial for the activation or repression of specific cellular processes. We provide support for pharmaceutical chemists focused on elucidating drug mechanisms of action, particularly how therapeutic agents interact with proteins and modify their function.
Workflow: Samples are lysed, alkylated and reduced, digested and purified prior nLC-HRMS/MS analysis. Pre-processing is performed with Proteome Discoverer (Thermo), data analysis is performed with in house script and algorithms.

Service Options:

• Bottom-up (analysis of peptides) proteomics
• Top-down (intact protein) proteomics